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cDNA synthesis-related question: Before beginning the reaction for cDNA synthesis, we need to incubate the extracted RNA molecules at a high temperature, like 65 degrees. What is this incubation for? Also, why is incubation in high temperature necessary for the reaction?Some mRNAs that are transcribed from histone genes or bacterial genes do not contain a polyadenylation tail. Also, damaged RNAs lose their polyadenylated tails during RNA extraction. How can you convert these RNAs that do not con

cDNA synthesis-related question: Before beginning the reaction for …

Synthesis of Luminol

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Fariha Naz on LinkedIn: #rtpcr #cdnasynthesis #geneexpression …

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