T7 RNA Polymerase - NEB
T7 RNA Polymerase is used for in vitro mRNA synthesis and is highly specific for the T7 phage promoter. Applications include: Radiolabeled RNA probe preparation. RNA generation for studies of RNA structure, processing and catalysis. Expression control via anti-sense RNA.
T7 RNA Polymerase - an overview | ScienceDirect Topics
The RNA polymerase from phage T7 (T7 RNAP) is a 98 000 Da molecular weight, single subunit whose polymerase domain is homologous to that of the DNA polymerase I family of polymerases and is completely unrelated to the multisubunit RNA polymerases. It initiates transcription at a specific site by binding to a 17-base-pair promoter DNA that it ...
An engineered T7 RNA polymerase that produces mRNA free of …
2022年11月10日 · For many research and commercial applications, IVT of mRNA is typically performed using bacteriophage T7 RNA polymerase (T7 RNAP) owing to its ability to produce full-length RNA transcripts...
Maximizing transcription of nucleic acids with efficient T7 …
2020年8月14日 · In vitro transcription using T7 bacteriophage polymerase is widely used in molecular biology. Here, we use 5′RACE-Seq to screen a randomized initially transcribed region of the T7 promoter...
The highly efficient T7 RNA polymerase: A wonder …
2018年10月15日 · The major gene product of T7 phage, T7 RNA polymerase (T7RNAP), continues to attract researchers since a long time due to its high and specific processivity with a single subunit structure and its capability of transcribing a …
Mechanism and Structure - Martin Lab - UMass
The consensus T7 RNA polymerase promoter, as defined by the 17 promoters in the T7 phage genome, extends from position -17 to about position +3, but see below. The cartoon above is shown in parallel with the initiation complex crystal structure below.
Fundamental Aspects of T7 RNA Polymerase Structure and …
Abstract. The enzymes that synthesize the nucleic acids — the polymerases — execute a reaction of fascinating complexity. Early work on these enzymes was carried out by biochemists who approached them from a classical, enzymological perspective and emphasized physical purity and quantitative assays (Korberg 1989).
T7 RNA Polymerase - Martin Lab - UMass
T7 RNA polymerase is widely used to synthesize RNA in vitro, from a DNA template. The single subunit RNA polymerase from bacteriophage T7 was initially characterized in 1973, but with its cloning and over expression in 1984, it became a work horse for …
A specific, promoter-independent activity of T7 RNA polymerase …
2018年9月17日 · This activity has been observed with T7 RNA polymerase (T7 RNAP), the well characterized prototype of the single subunit polymerases, as a nonspecific addition of nucleotides to the 3′ end of...
Fundamental Aspects of T7 RNA Polymerase Structure and Mechanism. R. SOUSA. 1 Introduction. The enzymes that synthesize the nucleic acids - the polymerases - execute a reaction of fascinating complexity.
- 某些结果已被删除