Stabilizing the closed SARS-CoV-2 spike trimer - Nature
2021年1月11日 · Stabilization of the hinge loop of the S proteins of SARS-CoV and MERS-CoV has been achieved by mutation of two consecutive residues in the S2 subunit between the …
A thermostable, closed SARS-CoV-2 spike protein trimer
2020年7月31日 · Here we have designed mutations in S that allow the production of thermostable, disulfide-bonded S-protein trimers that are trapped in the closed, prefusion …
Structural and functional properties of SARS-CoV-2 spike protein ...
2020年8月3日 · In this review, we summarize advances in research of the SARS-CoV-2 S protein and its therapeutic targeting. With a size of 180–200 kDa, the S protein consists of an …
Distinct conformational states of SARS-CoV-2 spike protein
Intervention strategies are urgently needed to control the SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) pandemic. The trimeric viral spike (S) protein catalyzes fusion …
Conformational dynamics of SARS-CoV-2 trimeric spike ... - Science
We report the cryo-EM structures of a tightly closed SARS-CoV-2 S trimer with packed fusion peptide and an ACE2-bound S trimer at 2.7- and 3.8-Å resolution, respectively. Accompanying …
A thermostable, closed SARS-CoV-2 spike protein trimer
We demonstrate that the designed, thermostable, closed S trimer can be used in serological assays. This protein has potential applications as a reagent for serology, virology and as an …
Structure of SARS-CoV-2 spike protein - ScienceDirect
2021年10月1日 · The trimeric spike (S) protein of the virus decorates the viral surface and is an important target for development of diagnostics, therapeutics and vaccines. Rapid progress in …
Simulation-driven design of stabilized SARS-CoV-2 spike S2
2024年8月27日 · Here, we use a simulation-driven approach to design S2-only immunogens stabilized in a closed prefusion conformation. Molecular simulations provide a mechanistic …
Integrative structural studies of the SARS-CoV-2 spike protein …
2022年1月1日 · Viral entry into host cells is facilitated by these S proteins forming trimer arrangements and binding to surface proteins of the host cell [4]. There is abundant structural …
The human coronavirus HCoV-229E S-protein structure and
2019年10月25日 · The HCoV-229E coronavirus S-protein accommodates extensive mutational change and possesses hydrophilic subunit interfaces in the S2 region, features that provide …