Biochemistry, Amino Acid Synthesis and Degradation
2023年1月11日 · Amino acids are organic compounds that consist of alpha carbon in the center, hydrogen (H), amino (-NH2), carboxyl (-COOH), and specific R (side chain) groups. One linear chain of amino acids is called a polypeptide; 1 or more polypeptides make up a protein.
1.17: Protein Structure - Biology LibreTexts
Each amino acid has the same core structure, which consists of a central carbon atom, also known as the alpha (α) carbon, bonded to an amino group (NH2), a carboxyl group (COOH), and a hydrogen atom.
Chemistry of Crosslinking | Thermo Fisher Scientific - US
EDC and other carbodiimides are zero-length crosslinkers; they cause direct conjugation of carboxylates (–COOH) to primary amines (–NH2) without becoming part of the final crosslink (amide bond) between target molecules.
Carbodiimide Crosslinker Chemistry - Thermo Fisher Scientific
Carbonyldiimidazole (CDI) can be used in non-aqueous conditions to activate carboxylic acids for direct conjugation to primary amines (–NH2) via amide bonds. Carbodiimide compounds provide the most popular and versatile method for labeling or crosslinking to carboxylic acids.
Distinct roles of the NH2- and COOH-terminal domains of the protein …
2000年5月9日 · A tyrosine-phosphorylated STAT forms a functional dimer through reciprocal Src homology 2 domain (SH2)-phosphotyrosyl peptide interactions. IFN treatment induces the association of PIAS1 and Stat1, which results in the inhibition of Stat1-mediated gene activation.
3.8: Proteins - Amino Acids - Biology LibreTexts
Each amino acid contains a central C atom, an amino group (NH2), a carboxyl group (COOH), and a specific R group. The R group determines the characteristics (size, polarity, and pH) for each type of amino acid. Peptide bonds form between the carboxyl group of one amino acid and the amino group of another through dehydration synthesis.
Distinct roles of the NH2- and COOH-terminal domains of the protein …
2000年5月5日 · The COOH terminus of PIAS1 can freely interact with unphosphorylated or phosphorylated Stat1. The presence of the NH 2 terminus of PIAS1 prevents the interaction of PIAS1 with a monomeric Stat1, probably because of steric interference. On cytokine stimulation, the dimerization of Stat1 through pTyr-701 and SH2 interaction results in a ...
Amidoligases with ATP-grasp, glutamine synthetase-like and ...
Here, the COOH-NH2 ligase PuuA condenses alpha-L-glutamate and putrescine by forming an amide linkage, which is followed by oxidation of the linked putrescine moiety to gamma-amino butyrate. Then PuuD, which is an amidohydrolase of the GAT-I superfamily (flavodoxin fold), hydrolyzes the isopeptide bond in gamma-glutamyl-gamma-aminobutyrate.
A General Protein Unfolding‐Chemical Coupling Strategy for Pure Protein …
2021年12月22日 · From a preparation viewpoint, we developed a new strategy to fabricate pure protein hydrogel with highly mechanical properties via combination of protein unfolding-gelation and -NH 2 /-COOH coupling reaction. The preparation strategy, in fact, reflects the unique network structures of this type of hydrogels, particularly for natural proteins.
2005年9月1日 · The role of the NH2 and COOH termini interaction on protein folding, function, stability, and turnover has been the subject of a recent study. Krishna and Englander (15) showed that the NH2 and the COOH termini of several transmembrane proteins interact and play a role in protein folding and stability.