Protein acylation: mechanisms, biological functions and …
2022年12月29日 · As the donor of protein lysine acetylation (Kace), acetyl-CoA is a central metabolite and substrate for anabolic metabolism, which has been studied thoroughly. It can be produced in the...
Repurposing a plant peptide cyclase for targeted lysine acylation
2024年5月24日 · Here we report a strategy for labelling lysine side chain ε-amines by repurposing an engineered asparaginyl ligase, which naturally catalyses peptide head-to-tail cyclization, for versatile...
Protein Lysine Acylation: Abundance, Dynamics and Function
2016年11月18日 · Underlining its importance in protein function, lysine undergoes a wide variety of PTMs including methylation, ubiquitination and acylation, which play a significant role in the regulation of protein properties. In this chapter, we focus on the post-translation N-acylation of proteins on internal lysines.
Lysine Acetylation Goes Global: From Epigenetics to Metabolism …
Lysine acetylation describes the transfer of an acetyl group from acetyl-coenzyme A (acetyl-CoA) to the primary amine in the ε-position of the lysine side chain within a protein, a process that leads to neutralization of the position’s positive electrostatic charge.
Lysine acylation using conjugating enzymes for site-specific ...
2020年9月14日 · Here we report a chemoenzymatic method, which we call lysine acylation using conjugating enzymes (LACE), to site-specifically modify folded proteins at internal lysine residues.
Lysine Acetylation Goes Global: From Epigenetics to Metabolism …
2018年2月6日 · Post-translational acetylation of lysine residues has emerged as a key regulatory mechanism in all eukaryotic organisms. Originally discovered in 1963 as a unique modification of histones, acetylation marks are now found on thousands of nonhistone proteins located in virtually every cellular compartment.
Chemical Biology Tools for Protein Lysine Acylation - PubMed
2022年5月16日 · Lysine acylation plays pivotal roles in cell physiology, including DNA transcription and repair, signal transduction, immune defense, metabolism, and many other key cellular processes. Molecular mechanisms of dysregulated lysine acylation are closely involved in the pathophysiological progress of ma …
Chemical Biology Tools for Protein Lysine Acylation
2022年3月18日 · Lysine acylation plays pivotal roles in cell physiology, including DNA transcription and repair, signal transduction, immune defense, metabolism, and many other key cellular processes. Molecular mechanisms of dysregulated lysine acylation are closely involved in the pathophysiological progress of many human diseases, most notably cancers.
Now open: Evolving insights to the roles of lysine acetylation in ...
2022年2月17日 · Acetylation of a specific lysine, K20, in PCNA by the cohesin KAT Eco1 (establishment of cohesion 1) is triggered by DNA damage to stimulate HR and suppresses translesion synthesis, thereby promoting DNA damage resistance in yeast. Structural studies indicate that acetylation alters the interfaces between PCNA molecules in the trimer.
An Expanding Repertoire of Protein Acylations - PMC
Acylation is one of the most common cotranslational covalent modifications of proteins in eukaryotes, which play a crucial role in regulation of protein function. The earliest discovered acylation was histone lysine acetylation (Kac) identified as a …