Guanidinium thiocyanate - Wikipedia
Guanidinium thiocyanate (GTC) or guanidinium isothiocyanate (GITC) is a chemical compound used as a general protein denaturant, being a chaotropic agent, although it is most commonly used as a nucleic acid protector in the extraction of DNA and RNA from cells.
Guanidinium Thiocyanate - an overview | ScienceDirect Topics
Guanidinium thiocyanate is a chaotropic agent used in protein degradation. The principle of this single-step technique is that RNA is separated from DNA after extraction with acidic solution consisting guanidinium thiocyanate and phenol.
Guanidine thiocyanate, 593-84-0, Molecular Biology, G9277, Sigma-Aldrich
Guanidine thiocyanate, or guanidinium rhodanide, is a versatile chemical compound widely used in molecular biology and biochemical research. Acting as a chaotropic agent, it disrupts cell and organelle membranes, facilitating cell lysis and organelle isolation.
Guanidine thiocyanate | CH5N3.CHNS | CID 65046 - PubChem
Guanidine thiocyanate | CH5N3.CHNS or C2H6N4S | CID 65046 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, safety/hazards/toxicity information, supplier lists, and more.
Guanidinium Thiocyanate - an overview | ScienceDirect Topics
Guanidinium thiocyanate (GuSCN) or guanidinium hydrochloride (GuHCl) are chaotropic salts that can be used to denature proteins and destroy nucleases that degrade DNA or adhere to the DNA (Bowtell, 1987; Cox, 1968).
Guanidine thiocyanate BioUltra, for molecular biology, 6M water …
Guanidine thiocyanate is a chaotropic agent that functions as a strong protein denaturant. It is widely used in RNA isolation protocols. Guanidine thiocyanate can irreversibly inactivate RNase and DNase and breaks cellular membranes.
The single-step method of RNA isolation by acid guanidinium thiocyanate ...
2006年6月27日 · The principle at the basis of the method is that RNA is separated from DNA after extraction with an acidic solution containing guanidinium thiocyanate, sodium acetate, phenol and chloroform,...
An Improved Method for High Quality Metagenomics DNA
2016年5月31日 · For lysis, first we treated the spheroplast with Guanidinium thiocyanate (GITC) to disrupt the bacterial cell membrane and inactivate nucleases and other enzymes.
methods that lyse pathogens and inactivate RNases with high concentrations of guanidinium salts. Herein, we investigate alternatives to standard guanidinium-based methods for RNase inactivation using an activity assay with an RNA substrate that fluoresces when cleaved.
Design of a hyperstable 60-subunit protein icosahedron
2016年7月7日 · Measurements were taken in the presence of Tris-buffered saline (TBS): 25mM Tris, 500mM NaCl; buffered guanidinium hydrochloride (GuHCl): 25mM Tris, 500mM NaCl, 1–6.7 M GuHCl; or buffered guanidine thiocyanate (GITC): 25mM Tris, 500mM NaCl, 1–4 M GITC.
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