A properly stained blood film is critical for malaria diagnosis, especially for precise identification of malaria species. Use of Giemsa stain is the recommended and most reliable procedure for staining thick and thin blood films. Giemsa solution is composed of eosin and methylene blue (azure). The eosin component stains
Giemsa Stain: Principle, Procedure, Results - Microbe Online
Giemsa is the most commonly used stain for staining blood films for malaria diagnosis. It is available commercially as a ready-to-use product, but the quality varies according to the source. By following simple rules, laboratories can prepare a stock solution of Giemsa stain using Giemsa stain powder, thus ensuring the use of consistent, high ...
Giemsa staining of malaria blood films - World Health Organization …
2016年1月1日 · The purpose of this document is to describe the procedure for properly staining malaria blood films with Giemsa stain. This document is also available in Arabic. This document is part of a series of 18 SOPs on malaria microscopy:
Giemsa Stain- Principle, Procedure, Results, Interpretation
2022年8月2日 · Giemsa stain is a gold standard staining technique that is used for both thin and thick smears to examine blood for malaria parasites, a routine check-up for other blood parasites and to morphologically differentiate the nuclear and cytoplasm of Erythrocytes, leucocytes and Platelets and parasites.
Malaria Diagnostic Tests | Malaria | CDC - Centers for Disease …
2024年4月24日 · (See DPDx specimen preparation) A blood specimen collected from the patient is spread as a thick or thin blood smear, stained with an appropriate stain (e.g. Giemsa, Wright-Giemsa), and examined with a 100X oil immersion objective. Visual criteria are used to detect malaria parasites and to differentiate (when possible) the various species.
To ensure that proper staining results have been achieved, if a positive smear (malaria) is available it may be included with each new batch of working Giemsa stain. Or the specimen being stained may be used as the
A freshly prepared working solution of Giemsa, made from well-prepared stock and diluted with water bu ered to pH 7.2 is recommended to achieve optimal staining of malaria blood films. Giemsa stock solution procured for national programmes is standardized to minimize frequent adjustments to SOPs on staining.
Global Malaria Programme - World Health Organization (WHO)
Microscopy is the method of choice for the investigation of malaria treatment failures. Giemsa is the recommended stain for malaria microscopy, and diagnosis requires examination of both thin and thick films from the same patient. Light microscopy is the diagnostic standard against which other diagnostic methods have traditionally been compared.
Appendix: Microscopic Procedures for Diagnosing Malaria
2005年5月31日 · For best staining results, blood films should be stained with a 2.5% Giemsa solution (pH of 7.2) for 45 minutes (alternate: 7.5% Giemsa for 15 minutes). Wright-Giemsa stain can also detect malaria parasites but does not demonstrate Schüffner's dots …
Giemsa staining of malaria blood films - iris.who.int
Giemsa staining of malaria blood films. View/ Open. WHO-HTM-GMP-MM-SOP-2016.07a-eng.pdf (79.99Kb) ...