Time kinetic analysis of H2AX phosphorylation by doxorubicin.
To investigate the potency of the topoisomerase II (topo II) poisons doxorubicin (Doxo) and etoposide (Eto) to stimulate the DNA damage response (DDR), Ser139 phosphorylation of …
Cytosolic accumulation of γH2AX is associated with ... - Nature
2008年6月20日 · DNA damage by doxorubicin and ionizing radiation induces autophosphorylation of ATM at Serine 1981 and subsequently activate multiple downstream targets such as p53, …
Early biomarkers of doxorubicin-induced heart injury in a ... - PubMed
2014年12月1日 · Male B6C3F1 mice were injected intravenously with 3mg/kg doxorubicin (DOX; an anti-cancer drug), or saline once a week for 2, 3, 4, 6, and 8weeks, resulting in cumulative …
FGF10 mitigates doxorubicin-induced myocardial toxicity in
2023年5月24日 · The phosphorylation of H2A histone family member X (H2AX) on serine 139 (called γ-H2AX) is the first step in recruiting DNA repair proteins, and γ-H2AX is a sensitive …
Gamma-H2AX immunofluorescence for the detection of tissue …
The phosphorylation of histone H2AX in Serine 139 (gamma-H2AX) marks regions of DNA double strand breaks and contributes to the recruitment of DNA repair factors to the site of DNA …
SIRT1 in the cardiomyocyte counteracts doxorubicin-induced ...
2022年3月8日 · We investigated whether SIRT1 counteracts doxorubicin-induced cardiotoxicity by mediating Ser139 phosphorylation of histone H2AX, a critical signal of the DNA damage …
Identification of a novel mechanism for reversal of doxorubicin …
2022年4月12日 · Given that phosphorylated γ-H2AX can quickly transduce DNA damage signals and trigger a series of biological cascade reactions [15], and γ-H2AX is a sensitive marker to …
γH2AX and Chk1 phosphorylation as predictive pharmacodynamic biomarkers ...
2014年7月4日 · To assess the specificity of pChk1 and γH2AX as biomarkers of cytotoxic chemotherapy plus V158411 combination activity, HT29 cells were treated with the …
DNA damage detected with gammaH2AX in endometrioid
Phosphorylation of histone H2AX (gammaH2AX) is a sensitive marker of DNA damage, particularly induction of DNA double-strand breaks. Using multiparameter cytometry we …
Treatment of cells with doxorubicin led to the phosphorylation of histone H2AX on serine 139 with dependence on ATM for the initial response.