Novel insights into peptide amidation and amidating activity …
2021年8月4日 · C-terminal α-amidation is the final and essential step in the biosynthesis of several peptide hormones. Peptidylglycine α-amidating monooxygenase (PAM) is the only known enzyme to catalyse this...
Enzymatic C-terminal amidation of amino acids and peptides
2012年7月18日 · Herein, we describe two versatile and high yielding enzymatic approaches for the conversion of semi-protected amino acid and peptidyl C-terminal α-carboxylic acids into their corresponding amides. In the first approach, the lipase Candida antarctica lipase-B (Cal-B), and in the second approach, the protease Subtilisin A, are used, respectively.
C-terminal modification and functionalization of proteins via …
2023年11月7日 · Herein, a chemical approach to modify the C-terminus is developed by fusing a cysteine protease domain on the C-terminus of the protein of interest, which could achieve the non-enzymatic...
Recombinant Production of Peptide C-Terminal α-Amides …
2013年10月19日 · Characteristic for these peptides is that they contain a C -terminal that is α-amidated, and this amidation is crucial for biological function. A challenge is to generate such peptides by recombinant means and particularly in a production scale. Here, we have examined an intein-mediated approach to generate a PYY derivative in a larger scale.
A Convenient Approach to Synthesizing Peptide C-Terminal N-Alkyl Amides
Here we report a convenient method to synthesize peptide C-terminal N-alkyl amides using the well-known Fukuyama N-alkylation reaction on a standard resin commonly used for the synthesis of peptide C-terminal primary amides, the PAL-PEG-PS (Peptide Amide Linker-polyethylene glycol-polystyrene) resin.
Mechanism of C-terminal amide formation by pituitary enzymes
1982年8月12日 · These observations indicate that biosynthesis of the C-terminal amide may involve the action of a specific enzyme which has a requirement for C-terminal glycine. We present here direct evidence...
A Biomimetic C-Terminal Extension Strategy for …
2023年8月31日 · Here, we report a biomimetic caging strategy in which the neuropeptide C-terminus is extended via a photocleavable amino acid to mimic the proneuropeptides found in large dense-core vesicles. We explored this approach with four prominent neuropeptides: gastrin-releasing peptide (GRP), oxytocin (OT), substance P (SP), and cholecystokinin (CCK).
C-Terminally modified peptides - RSC Publishing
2016年1月26日 · A large variety of C-terminally modified peptides was obtained by nucleophilic cleavage of the ester bond in solid phase linked peptide esters of 4-hydroxymethyl benzamide (HMBA). The developed methods provided peptides, C-terminally functionalized as esters, amides and thioesters, with high purity directly from the resin in a single reaction step.
C-terminal modification of monoclonal antibody drugs: Amidated species ...
2013年1月1日 · This study showed that C-terminal amidation is a general C-terminal modification on the heavy chains of therapeutic mAbs and that C-terminal amidation of mAbs can be catalyzed by a certain PAM(s) in the Chinese hamster ovary (CHO) cells that are widely used for manufacturing therapeutic mAbs.
A Comparison of the Effects of Amide and Acid Groups at the C-Terminus ...
2012年7月26日 · In the peptide amides, the carboxylic acid group at the C-terminus is replaced with a less acidic terminal amide group. A comparison of the gas-phase acidities (GAs) of simple compounds like acetic acid and acetamide reveals a distinct difference in the energy required for the deprotonation of carboxylic acid versus amide functional groups.
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