Automated Sequencing - an overview | ScienceDirect Topics
Automation of DNA Sequencing. Today, virtually all DNA sequencing uses automated sequencers. Although the principle is the same, but the method of detection is different. For automated sequencing, either the primer or the ddNTPs are labeled by incorporation of a fluorescent dye (Fig. 28.3 (a)).
DNA sequencing - Wikipedia
DNA sequencing is the process of determining the nucleic acid sequence – the order of nucleotides in DNA. It includes any method or technology that is used to determine the order of the four bases: adenine, guanine, cytosine, and thymine.
DNA Sequencing Technologies | Learn Science at Scitable - Nature
In 1986, a company named Applied Biosystems began to manufacture automated DNA sequencing machines based on the Sanger method (Figure 1). These machines used fluorescent dyes to tag each...
Next-generation DNA sequencing | Nature Biotechnology
2008年10月9日 · Next-generation DNA sequencing has the potential to dramatically accelerate biological and biomedical research, by enabling the comprehensive analysis of genomes,...
Sequencing Genomes - Genomes - NCBI Bookshelf
Describe the key features of automated DNA sequencing and evaluate the importance of automated sequencing in genomics research. State the strengths and limitations of the shotgun, whole-genome shotgun and clone contig methods of genome sequencing.
Sequencing - Mapping and Sequencing the Human Genome - NCBI Bookshelf
The nucleotide sequence of a genome is its physical map at the highest level of resolution. It provides all the information that goes into making up an individual's genetic complement, and no two individuals (except identical twins) share the same genome sequence.
DNA sequencing at 40: past, present and future | Nature
2017年10月11日 · This review commemorates the 40th anniversary of DNA sequencing, a period in which we have already witnessed multiple technological revolutions and a growth in scale from a few kilobases to the...
Digital-WGS: Automated, highly efficient whole-genome sequencing …
2020年12月9日 · Single-cell whole-genome sequencing (WGS) is critical for characterizing dynamic intercellular changes in DNA. Current sample preparation technologies for single-cell WGS are complex, expensive, and suffer from high amplification bias and errors.
DNA Sequencing: Methods and Applications | SpringerLink
2013年10月22日 · 2.3.3 Automated DNA Sequencing Methods. The principle of automated DNA sequencing is same as Sanger’s method but the detection is different. In this automated method, the primer or the ddNTPs are labeled by incorporation of a fluorescent dye.
Evolution of Genetic Techniques: Past, Present, and Beyond
Manual Sanger sequencing method has been improved by the introduction of first generation of automated DNA sequencers . Automatization of DNA sequencing enabled sequencing human genome in a fast and accurate way. With the advances in the field of molecular genetics, it became possible to launch the Human Genome Project to reveal the complete ...