1.1 Strep-Tactin XT:Twin-Strep-tag system overview The newest generation of the Strep-tag® system is a further development of the commonly used Strep-Tactin®:Strep-tag®II technology. The Strep-tag®II is a short peptide, which binds with high selectivity to Strep-Tactin®, an engineered streptavidin. This technology allows one-
Strep-tag® technology - One affinity tag for all applications
Strep-tag® technology for cell and protein isolation. It is characterized by high purity, full functionality of the target material, simple purification steps and reversibility of tag-ligand binding.
Strep•Tag® II Fusion Tag Provides Rapid One-Step
Rapid, one-step affinity purification of Strep•Tag ® fusion proteins can result in active proteins at greater than 95% purity. In a comparison of affinity tags, the Strep•Tag peptide was shown to provide excellent purification, with good yields, at a moderate cost (1).
Monovalent Strep-Tactin for strong and site-specific tethering in ...
Here, we introduce a monovalent form of Strep-Tactin that harbours a unique binding site for Strep-tag II and a single cysteine that allows Strep-Tactin to specifically attach to the atomic force microscope cantilever and form a consistent pulling geometry to …
The Strep-tag®II/Strep-Tactin® interaction is compatible with a variety of reagents (see Table 1) making the system attractive for purifing metallo- and membrane proteins, large proteins and protein complexes.
Strep-Tactin®XT technology - IBA Lifesciences
Strep-Tactin®XT is the high affinity variant for the purification of strep-tagged fusion proteins, providing binding affinities in the picomolar range for Twin-Strep-tag® while still maintaining binding reversibility and mild recovery of immobilized proteins.
Monovalent Strep -Tactin for strong and site-specific ... - Nature
2015年10月12日 · Here, we introduce a monovalent form of Strep -Tactin that harbours a unique binding site for Strep -tag II and a single cysteine that allows Strep -Tactin to specifically attach to the atomic...
The Strep-tag system for one-step purification and high-affinity ...
2007年6月14日 · We describe a protocol that enables quick and mild purification of corresponding Strep -tag II fusion proteins—including their complexes with interacting partners—both from bacterial and...
Strep-Tactin® for reversible binding of biotinylated or Strep …
The reversible binding of Strep-Tactin® offers many applications in characterization of protein complexes and mapping of protein-protein interactions using AP-MS or proximity-dependent biotin identification (BioID).
Strep -Tactin Superflow Plus - QIAGEN
Strep-Tactin Superflow Plus allows highly efficient purification of proteins (see figures Efficient purification of Strep-tagged GFP with Strep-Tactin Superflow Plus and Highly specific detection of Strep-tagged proteins in any expression system).