A bacterial proteorhodopsin proton pump in marine eukaryotes
2011年2月8日 · To determine the subcellular location of the Group 1 proteorhodopsin in O. marina, an antibody was developed against the 14 C-terminal residues shared by several …
Lipid Bilayer Composition Can Influence the Orientation of ...
Antibody-binding assay and proteolysis of proteoliposomes showed physical evidence of preferential orientation, and functional assays verified the vectorial nature of ion transport in …
Scramblase activity of proteorhodopsin confers physiological …
2023年12月15日 · Here, we report the discovery of light-independent function of a proteorhodopsin from a psychrophile Psychroflexus torquis (ptqPR). ptqPR could improve the …
Proteorhodopsin expression and characterization. (A) Western …
Proteorhodopsin (PR) is a light harvesting protein widely distributed among bacterioplankton that plays an integral energetic role in a new pathway of marine light capture.
The proteorhodopsins of the dinoflagellate Oxyrrhis marina ...
2020年7月3日 · No green immunofluorescence was detected when the Alexa488 labeled antibody was used alone (Fig. 7b), whereas an intense labeling of the cell periphery was registered …
Cellular localization of proteorhodopsin in O. marina cells. (a ...
Antibody signal forms small irregular and ring-like structures independent of mitochondria in O. marina. Three independent cells are shown, each showing (left to right) differential interference...
The proteorhodopsins of the dinoflagellate Oxyrrhis marina ...
Aliquots of those proteorhodopsin fractions were used for negative staining transmission electron microscopy, freeze-fracture transmission electron microscopy, Cryo-TEM, immunoelectron …
Function and Regulation of Vibrio campbellii Proteorhodopsin
2012年6月7日 · In this study, we describe the presence and function of a horizontally acquired PR and retinal biosynthesis gene cluster in the culturable and genetically tractable bioluminescent …
Heterologous expression and cell membrane localization of ...
2020年6月9日 · On the Western blot, the antibody recognized a single, highly abundant band of the predicted size, 30 kDa (Fig. 2a, b). Because Flag gene was placed downstream of …
Proteorhodopsin is a Light-driven Proton Pump with Variable ...
2002年8月30日 · Our results show that proteorhodopsin is a light-driven proton pump with characteristics similar to those of BR at alkaline pH. However, at acidic pH, the direction of …