Efficient prime editing in two-cell mouse embryos using PEmbryo …
2024年2月6日 · Using transient inhibition of DNA mismatch repair during a permissive stage of development, we demonstrate highly efficient prime editing of mouse embryos with few unwanted, local byproducts ...
Generation of mouse–human chimeric embryos - Nature
2021年7月2日 · The protocol is suitable for studying the development of hPSCs in mouse embryos and may facilitate the generation of human cells, tissues and organs in animals.
Embryo Microinjection With CRISPR-Cas9 in Mice and Zebrafish
To test the efficiency of CRISPR-Cas9–mediated genome editing in mouse models using either Cas9 recombinant protein, or Cas9 mRNA and in vitro–transcribed short guide RNA (sgRNA), we perform pronuclear injections and culture the zygotes to the blastocyst stage. We term this the “blastocyst assay”.
Efficient generation of targeted large insertions by ... - Nature
2018年6月11日 · Rapid, efficient generation of knock-in mice with targeted large insertions remains a major hurdle in mouse genetics.
CATI: an efficient gene integration method for rodent and primate ...
2023年6月23日 · Using CasRx to downregulate a key MMEJ factor, Polymerase Q (Polq), we improve the targeted integration efficiency of linearized DNA fragments and single-strand oligonucleotides (ssODN) in mouse embryos and offspring.
Contribution of Mouse Embryonic Stem Cells and Induced …
2014年12月28日 · Unlike embryonic stem cells, the mouse chimeras were only generated from injection of 8-cell embryos with induced pluripotent stem cells and none of these showed germline transmission.
Determining the potency of primordial germ cells by injection …
2024年3月25日 · Following injection into early mouse embryos, PGCs can display properties akin to naive pluripotent cells, but they do not functionally contribute to later embryogenesis, supporting the notion that PGCs exhibit a latent form of pluripotency.
Automated mouse embryo injection moves toward practical use
Further improvement of the automated system will change the way of how mouse embryos are injected and promise its practical use in biology laboratories and mouse facilities.
Producing fully ES cell-derived mice from eight-cell stage embryo ...
In conventional methods for the generation of genetically modified mice, gene-targeted embryonic stem (ES) cells are injected into blastocyst-stage embryos or are aggregated with morula-stage embryos, which are then transferred to the uterus of a surrogate mother.
Blastocyst Microinjection with Embryonic Stem Cells
Blastocysts are commonly used as the host embryos for this purpose, as it is relatively easy to inject cells into the blastocoel cavity of the developing embryo. This chapter describes the procedure for injection of ES cells into blastocyst stage …