Investigating mitochondrial autophagy by routine transmission …
2020年10月1日 · One technique to monitor mitophagy is transmission electron microscopy (TEM). TEM images can provide valuable information on the ultrastructure of the organelle, the integrity of the inner and outer mitochondrial membrane (IMM and OMM), changes in mitochondrial size and mitochondria-ER interaction preceding mitophagy.
Mitochondrial Morphology and Mitophagy in Heart Diseases
2021年5月6日 · Recently, the cardiovascular TEM enabled the studying of several signaling processes impacting mitochondrial function, such as mitochondrial fission/fusion, autophagy, mitophagy, lysosomal degradation, and lipophagy.
Mitochondrial Morphology and Mitophagy in Heart Diseases: …
Recently, the cardiovascular TEM enabled the studying of several signaling processes impacting mitochondrial function, such as mitochondrial fission/fusion, autophagy, mitophagy, lysosomal degradation, and lipophagy.
Frontiers | Mitochondrial Morphology and Mitophagy in Heart …
2021年5月5日 · Mitophagy is the specific autophagic degradation of mitochondrial cargo and is mediated by Pink1–Parkin-dependent and -independent pathways. TEM was used to examine cardiac ultrastructure in hearts of the Parkin KO mice under basal conditions and in the response to MI (Kubli et al., 2013). Parkin KO mice have smaller body weight (BW) and ...
Semi-automated quantitation of mitophagy in cells and tissues
In this methods article we describe step-by-step how to detect and quantify mitophagy and show that mitophagy levels can be reliably calculated in different cell lines and under distinct stimuli. Finally, we show that the mito-QC Counter can be used to quantify mitophagy in tissues of mito -QC transgenic mice.
Mechanisms of mitophagy in cellular homeostasis, physiology …
2018年8月28日 · Mitophagy is an evolutionarily conserved cellular process to remove dysfunctional or superfluous mitochondria, thus fine-tuning mitochondrial number and preserving energy metabolism.
Correlative Light and Transmission Electron Microscopy Showed …
2020年9月29日 · Until now, conventional techniques for analysis of mitophagy have been based on immunofluorescence staining and immunoblotting of several specific mitochondrial proteins, qPCR for mitochondrial DNA copy number, and nano-resolution imaging using transmission electron microscopy (TEM).
Mitophagy in the Pathogenesis of Liver Diseases - PMC
2020年3月30日 · Recent studies have shown that mitophagy may participate in the pathogenesis of various liver diseases, such as liver injury, liver steatosis/fatty liver disease, hepatocellular carcinoma, viral hepatitis, and hepatic fibrosis.
Systematic Transmission Electron Microscopy‐Based Identification …
2023年3月3日 · These LDs can protect against ER stress and may protect against mitochondrial autophagy, known as mitophagy, by forming close mitochondria-to-lipid contacts. Autophagy impacts every cellular organelle due to its role in organelle degradation.
Longitudinal autophagy profiling of the mammalian brain reveals ...
2024年10月4日 · Mitophagy and macroautophagy are dynamic processes that exhibit cell and region-specific changes throughout mammalian life. While mitophagy increases throughout lifespan in some cell types e.g., Purkinje neurons, decreases are observed in others, for instance in the dentate gyrus.
- 某些结果已被删除