CCL5-deficiency enhances intratumoral infiltration of CD8
2018年7月10日 · c The tumor growth curves of CT26 shNTC and CT26 shCCL5 tumor cells were injected into the back of female WT or KO mice treated with CD8 T-cell-depleting antibody. Tumor growth was monitored every ...
SET facilitates immune escape of microsatellite stability colorectal ...
2024年10月17日 · The human MSS CRC (HT29) and murine MSS CRC (CT26) cell lines were obtained from the China Center for Type Culture Collection (Wuhan, China). ... Representative photographs (×200, scale = 50 μm) showing IHC analysis of SET and CD8 (left) and correlation between the proportion of SET-positive and proportion of CD8-positive area (right) in ...
Interferon-γ in the tumor microenvironment promotes the
2024年3月13日 · After that, CD8 + T cells were treated with recombinant human IL-2 (peprotech, 200-02, 100 U/ml).CD8 + T cells were co-cultured with CT26-B7H4-OVA for 24 h, and then cells were analysis by FC. IHC
Tumor-immune profiling of CT-26 and Colon 26 syngeneic mouse …
2021年11月13日 · Though the frequency of Tregs (CD45+ CD3+ CD11b- CD4+ CD8- CD25+ Foxp3+) was also higher in CT-26 tumor tissues, (Fig. 2G), the ratio of CD8 to Treg in CT-26 tumor tissues was significantly higher than that in Colon 26 tumor tissues (Fig. 2H). Our data showed that CT-26 tumor tissues exhibited immune-inflamed phenotype characterized by more ...
Different syngeneic tumors show distinctive intrinsic tumor …
2022年2月28日 · In three syngeneic tumors (MC38, CT26 and EMT6) where CD8 +-CTL-TILs seem to be directly responsible for anti-PD-1 mediated tumor inhibition, CD8 +-TIL depletion combined with anti-PD-1 treatment ...
Comparison of the molecular and cellular phenotypes of common …
2020年1月2日 · CT26, a colon tumor model, had the highest immunogenicity and was the model most responsive to CTLA4 inhibitor treatment, by contrast to the relatively low immunogenicity and response rate to checkpoint inhibitor therapies in human colon cancers. ... (IHC) features of a panel of ten commonly used mouse syngeneic models [6, 10, 13] with the ...
MAP7D2 reduces CD8+ cytotoxic T lymphocyte infiltration …
2023年1月4日 · (A) The workflow of anti-PD-1 therapy was shown. (B–D) CT26 cells were subcutaneously implanted into BALB/c mice and received anti-PD-1 therapy. The tumor growth curves and tumor weight are shown. (E and F) IHC staining of CD3 and CD8 to evaluate CD3 + and CD8 + T cells infiltrated in CT26 subcutaneous tumors after receiving anti-PD-1 therapy ...
MAP7D2 reduces CD8 - Cell Press
2023年1月4日 · (A) The workflow of anti-PD-1 therapy was shown. (B–D) CT26 cells were subcutaneously implanted into BALB/c mice and received anti-PD-1 therapy. The tumor growth curves and tumor weight are shown. (E and F) IHC staining of CD3 and CD8 to evaluate CD3 + and CD8 + T cells infiltrated in CT26 subcutaneous tumors after receiving anti-PD-1 therapy ...
Long-lasting mRNA-encoded interleukin-2 restores CD8 - Cell Press
2024年3月14日 · B2M is the shared component of all MHC class I molecules. Its loss results in complete absence of MHC class I surface expression and consequently the lack of CD8 + T cell recognition. To study MHC class I presentation deficiency in different settings, we deleted B2m genes in three mouse tumor cell lines. Of these, CT26 and MC38 form highly immunogenic tumors and elicit spontaneous CD8 + T cell ...
CXCL8 induces M2 macrophage polarization and inhibits CD8
2023年9月4日 · CXCL8/CT26 or control/CT26 was subcutaneously inoculated into each mouse (male, 4 weeks). Seven days later, ... Representative IHC images of CXCL8, CD3, and CD8 were analyzed in CXCL8 low-expression group (upper) and CXCL8 high-expression group (lower) from clinical paraffin tissue chip. ...