Thus, small- to large-scale transient transfection of mammalian cells growing in suspension is needed. However, because of the very high cost of transfection reagents, adoption of this modern ...

Change the medium 1 h before transfection ... 3| Immediately transfer the calcium phosphate–DNA suspension into the medium above the cell monolayer. Use 0.1 ml of suspension for each 1 ml ...

3D suspension culture is a promising solution for large-scale, high-quality pluripotent stem cell (PSC) production for cell therapy manufacturing. Gibco™ CTS™ StemScale™ PSC Suspension Medium ...