The initial steps in preparation of biological material for scanning electron microscopy (SEM) are similar to specimen preparation for TEM. After dehydration into absolute ethanol, specimens are dried ...

While TEM can offer a higher resolution than SEM, it requires a more complicated sample preparation process and lacks the three-dimensional imaging capabilities of SEM. REM uses low-energy electrons ...

That's why Jiro Usukura, at the Structural Biology Research Center, Nagoya University, says good sample preparation technique is essential. "The effort of producing high-resolution cryo-EM images ...