Primary protein purification is automated and integrated into the robotics platform and uses cell lysis, high-speed centrifugation and affinity purification. The initial results from this system ...

our findings show that it will be possible to increase the yield for many His-tagged proteins and enable IMAC purification for very low-abundance proteins. We believe that our method could greatly ...

Suitable antibodies for immunoprecipitation of the desired protein complex and optimization of the cell line, culture conditions and methods for cell lysis and antibody affinity purification are ...

Purification of DNA from cell extract In addition to DNA the cell extract will contain significant quantities of protein and RNA. A variety of procedures can be used ... chemical digestion is ...