at the selected NMR protein concentration, acquiring 1D 1 H and/or 2D 15 N HSQC spectra. This will take about 1 day, depending on sample concentration. These preliminary data will allow us to evaluate ...
Structure determination by NMR typically requires a protein concentration of 0.5 mM or greater, stable for several days at the desired temperature, usually 20-40 o C. Proteins and peptides should be ...