To decipher how this occurs, the authors developed an in vitro assay to look for triggers of ... The authors propose “that PDI is a chaperone that changes its affinity for substrates during ...

In addition to these established techniques, new assays are critically needed to test chaperone mechanisms in live cells. One possibility is to design in-vivo protein-folding biosensors, which could ...

Cyclophilins are a family of protein chaperones and signaling molecules found in every domain of life. In humans, they play key roles in numerous signaling pathways and contribute to several ...

Chaperone proteins surround a protein during the folding process, sequestering the protein until folding is complete. For example, in bacteria, multiple molecules of the chaperone GroEL form a ...

This work will be of high interest to a broad swathe of scientists studying native macromolecules present at low concentrations in cells. Cryo-EM single-particle analyses typically require target ...