Most chemical enhancements produce a moderate increase in gene delivery and a limited increase in gene expression 1. As a result, the efficiency of transfection and level of gene expression after ...

1| Harvest exponentially growing cells 24 h before transfection by trypsinization and replate them at a density of 1 × 10 5 to 4 × 10 5 cells/cm 2 in 60-mm tissue culture dishes or in 12-well ...