Change the medium 1 h before transfection. 2| To prepare the calcium phosphate–DNA coprecipitate, combine 100 μl of 2.5 M CaCl 2 with 25 μg of plasmid DNA in a sterile 5-ml plastic tube and ...
When it meets up with oxygen, it becomes phosphate. Most phosphates team up with calcium to build and repair your bones and teeth. Some support your muscles and nerves. Here's where things get ...