Commercial reagent kits are available for bisulfite treatment of human genomic DNA. The PCR is performed with one of the PCR primers biotinylated. This is required for the conversion of the PCR ...

2009;9(3):243-257. The fluorogenic amplification reactions are usually carried out in triplicate in a volume of 20 µl that contained 3 µl of bisulfite-modified DNA; 600 nM concentrations of ...